10. Using the following DNA template strand sequence, answer the questions that follow. (10)
10a. What is the complementary DNA strand sequence? (Use only the template sequence in
106. Now write down the sequence of the corresponding m-RNA. (Use only the template
sequence in bold letters.)
Biology 204: Principles of Biology I (Rev. C8)
Assignment 4 A
10c. What is the sequence of amino acids in the peptide produced?
AAA-GUG-UCG-UCA-CUG-GUA, and the the corresponding amino acid sequence is lys-
Suppose the DNA sequence becomes mutated and cytosine is inserted before position 11 on
the template strand (for orientation, TTT occupy positions 1, 2 and 3 respectively).
10d. Write down the sequence of the mutated DNA, the sequence of the corresponding m-
RNA and the resulting peptide that is produced as a result of the mutation. (Hint: the
start codon remains intact.)
The mutated sequence is AAAGUGUCGUCGACUGGUA and would translate to the
polypeptide chain !ys-val-ser-ser-thr-gly.
10e. What kind of a mutation is this?
This form of mutation is called a frameshift mutation. A frameshift mutation is when
base pair is added or deleted in the coding sequence, which alters the reading frame of
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